Novel identification of biofluids using a multiplex methylation sensitive restriction enzyme-PCR system
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- 作者：Yu-Chih Lina ; b ; Li-Chin Tsaic ; James Chun-I Leed ; Chih-Wen Suc ; e ; Jason Tze-Cheng Tzena ; Adrian Linacref ; Hsing-Mei Hsieh ; Ph.Dc ; mei@mail.cpu.edu.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：Biofluid identification ; Biofluid-specific methylated marker ; Methylation sensitive restriction enzyme-PCR (MSRE-PCR) ; miniSTR
- 刊名：Forensic Science International: Genetics
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：25
- 期：Complete
- 页码：157-165
- 全文大小：1810 K
文摘
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A new 10-plex MSRE-PCR system was established to identify the body fluids of venous blood, semen, saliva, vaginal fluids and menstrual blood.
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The validation of the 10-plex MSRE-PCR system showed that 0.25 ng DNA was sufficient to obtain reproducible identification of biofluid types.
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The MSRE-PCR system and AmpFlSTR® MiniFiler™ were co-amplified in a single reaction to generate both biofluid and miniSTR profiles.