Effect of low water temperature on viral replication of white spot syndrome virus in Procambarus clarkii

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• 作者：Huahua Du ; Wei Dai ; Xinyan Han ; Weifen Li ; Yaxiang Xu ; Zirong Xu
• 关键词：White spot syndrome virus ; WSSV ; Viral replication ; Water temperature ; Procambarus clarkii
• 刊名：Aquaculture
• 出版年：2008
• 出版时间：3 June 2008
• 年：2008
• 卷：277
• 期：3-4
• 页码：149-151
• 全文大小：377 K

文摘

This study evaluated the effect of low water temperature (10 ± 1 °C) on viral infection and replication of white spot syndrome virus (WSSV) in crayfish, Procambarus clarkii, under standardized conditions. Crayfish were (i) maintained at 24 ± 1 °C before challenge and 10 ± 1 °C afterwards, or (ii) maintained at 10 ± 1 °C before challenge and 24 ± 1 °C afterwards. No mortality was observed when crayfish were held at 10 ± 1 °C after challenge, but mortality reached 100 % when they were transferred to 24 ± 1 °C. Competitive PCR showed that viral levels at 10 ± 1 °C rose from 10⁶ to 10⁸ copies/mg of gill tissues, while at 24 ± 1 °C levels increased from 10⁶ to 10¹⁰ copies/mg of gill tissues during the same time interval. These results showed that a low water temperature of 10 ± 1 °C could reduce viral replication when compared to 24 ± 1 °C but could not prevent it.