Limb ischemic preconditioning mitigates lung injury induced by haemorrhagic shock/resuscitation in rats

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• 作者：Woan-Ching Jan^a ; Cay-Huyen Chen^b ; ^c ; Pei-Shan Tsai^d ; Chun-Jen Huang^b ; ^c ; ^{huangcj1112@gmail.com"" rel=""nofollow}
• 关键词：Ischemia/reperfusion ; Heme oxygenase ; MIP-2 ; IL-6 ; Nitric oxide
• 刊名：Resuscitation
• 出版年：2011
• 出版时间：June 2011
• 年：2011
• 卷：82
• 期：6
• 页码：760-766
• 全文大小：929 K

文摘

Aim of the study

Haemorrhagic shock and subsequent resuscitation induce acute lung injury. We elucidated whether bilateral lower limb ischemic pre-conditioning (IP) could mitigate lung injury in haemorrhagic shock/resuscitation rats. The role of heme oxygenase-1 (HO-1) was also elucidated.

Method

Adult male rats were randomized to receive haemorrhagic shock/resuscitation (HS), HS plus IP, or HS plus IP plus the HO-1 inhibitor tin protoporphyrin (SnPP) (n = 12 in each group). Sham groups were employed simultaneously. For pre-conditioning, 3 cycles of limb IP (10 min ischemia followed by 10 min reperfusion) were performed immediately before haemorrhagic shock. Haemorrhagic shock (mean arterial pressure: 40–45 mmHg) was induced by blood drawing and maintained for 120 min. SnPP was injected 5 min before resuscitation. Shed blood/saline mixtures were re-infused to achieve resuscitation. After monitoring for another 8 h, rats were sacrificed. Arterial blood gas and alveolar–arterial oxygen difference (lung function index), histology, polymorphonuclear leukocytes/alveoli ratio (leukocyte infiltration index), wet/dry weight ratio (water content index), inflammatory molecules (e.g., chemokine, cytokine, prostaglandin E₂), and malondialdehyde (lipid peroxidation index) assays were preformed.

Results

Haemorrhagic shock/resuscitation induced significant lung function alterations and significant increases in leukocyte infiltration, water content, inflammation, and lipid peroxidation in lungs. Histological analysis confirmed that haemorrhagic shock/resuscitation caused marked lung injury. Limb IP significantly mitigated the adverse effects of haemorrhagic shock/resuscitation. Moreover, the protective effects of limb IP were reversed by SnPP.

Conclusions

Limb IP mitigates lung injury in haemorrhagic shock/resuscitation rats. The mechanisms may involve HO-1.