Magnesium Sulfate Mitigates Lung Injury Induced by Bilateral Lower Limb Ischemia-Reperfusion in Rats

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• 作者：Ming-Chang  ; Kao M.D.^&lowast ; ;   ; ^&dagger ; ; Woan-Ching  ; Jan Ph.D.^&Dagger ; ; Pei-Shan  ; Tsai Ph.D.^§ ; ; Tao-Yeuan  ; Wang M.D.^?/sup> ; Chun-Jen  ; Huang MD ; Ph.D.^&lowast ; ;   ; ^&dagger ; ;   ; ^{huangcj1112@gmail.com}
• 关键词：MIP-2 ; IL-6 ; NO ; MDA ; L-type calcium channels
• 刊名：Journal of Surgical Research
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：171
• 期：1
• 页码：e97-e106
• 全文大小：1596 K

文摘

Background

Lower limb ischemia-reperfusion (I/R) elicits oxidative stress and causes inflammation in lung tissues that may lead to lung injury. Magnesium sulfate (MgSO₄) possesses potent anti-oxidation and anti-inflammation capacity. We sought to elucidate whether MgSO₄ could mitigate I/R-induced lung injury. As MgSO₄ is an L-type calcium channel inhibitor, the role of the L-type calcium channels was elucidated.

Materials and Methods

Adult male rats were allocated to receive I/R, I/R plus MgSO₄ (10, 50, or 100 mg/kg), or I/R plus MgSO₄ (100 mg/kg) plus the L-type calcium channels activator BAY-K8644 (20 ¦Ìg/kg) (n = 12 in each group). Control groups were run simultaneously. I/R was induced by applying rubber band tourniquets high around each thigh for 3 h followed by reperfusion for 3 h. After euthanization, degrees of lung injury, oxidative stress, and inflammation were determined.

Results

Arterial blood gas and histologic assays, including histopathology, leukocyte infiltration (polymorphonuclear leukocytes/alveoli ratio and myeloperoxidase activity), and lung water content, confirmed that I/R caused significant lung injury. Significant increases in inflammatory molecules (chemokine, cytokine, and prostaglandin E₂ concentrations) and lipid peroxidation (malondialdehyde concentration) confirmed that I/R caused significant inflammation and oxidative stress in rat lungs. MgSO₄, at the dosages of 50 and 100 mg/kg but not 10 mg/kg, attenuated the oxidative stress, inflammation, and lung injury induced by I/R. Moreover, BAY-K8644 reversed the protective effects of MgSO₄.

Conclusions

MgSO₄ mitigates lung injury induced by bilateral lower limb I/R in rats. The mechanisms may involve inhibiting the L-type calcium channels.