Utilization of agro-industrial wastes for the production of lipase from Stenotrophomonas maltophilia isolated from Arctic and optimization of physical parameters
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- 作者：C.S. Neethu ; neethucs_1985@yahoo.co.in" class="auth_mail" title="E-mail the corresponding author ; K.M. Mujeeb Rahiman ; Emilda Rosmine ; A.V. Saramma ; A.A. Mohamed Hatha
- 关键词：Lipase ; Agro-industrial waste ; Stenotrophomonas maltophilia ; Optimization
- 刊名：Biocatalysis and Agricultural Biotechnology
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：4
- 期：4
- 页码：703-709
- 全文大小：1143 K
文摘
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A potential cold tolerant lipase producer Stenotrophomonas maltophilia was isolated from Arctic sediment.
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Lipase was successfully produced on agro-industrial wastes groundnut cake, coconut cake, neem cake, wheat bran and rice bran and ground nut was found to be superior substrate for lipase production.
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Temperature, pH, and moisture content were optimized for all substrates tested.
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Enzyme concentrated by acetone precipitation was purified to 2.9 fold by DEAE-Cellulose.
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Molecular weight of the purified lipase was found to be 49.1 KDa.