- 作者：Val¨¦rie Gu¨¦rin-El Khourouj ; Marie Duchamp ; Anne Krivine ; B¨¦atrice P¨¦dron ; Marie Ouach¨¦e-Chardin ; Karima Yakouben ; Marie-Louise Fr¨¦mond ; Andr¨¦ Baruchel ; Jean-Hugues Dalle ; Ghislaine Sterkers
- 关键词：HSCT ; hematopoietic-stem cell transplantation ; GVHD ; graft versus host disease ; TIV ; trivalent inactivated seasonal vaccine ; PBMC ; peripheral blood mononuclear cells ; AdV ; adenovirus ; Cpm ; counts per minute ; SI ; stimulation index ; HA ; hemagglutinin ; NA ; n
- 刊名：Human Immunology
- 出版年：2012
- 出版时间：September, 2012
- 年：2012
- 卷：73
- 期：9
- 页码：884-890
- 全文大小：454 K
Here, 14 pediatric recipients (mean age: 6 years) received H1N1 (n = 9) or H1N1/H3N2 (n = 5) vaccines at a median of 5.7 months post-HSCT (HLA-identical related bone-marrow graft: 10/14). Fourteen clinically-matched non-vaccinated recipients were included as controls. Cellular response to vaccination was assessed by a T-cell proliferation assay. Humoral response was assessed by H1N1-specific antibody titration. IL2 and IFN¦Ã responses to influenza were also evaluated by an intracellular cytokine accumulation method for some of the recipients.
Higher proliferative responses to H1N1 (p = 0.0001) and higher H1N1-specific antibody titers (p < 0.02) were observed in vaccines opposed to non-vaccinated recipients. In some cases, proliferative responses to H1N1 developed while at the same time antibody titers did not reach protective (?1:40) levels. Most recipients vaccinated with only the H1N1 strain had proliferative responses to both H1N1 and H3N2 (median stimulation index H1N1: 96, H3N2: 126 in responders). Finally, IL2 responses predominated over IFN¦Ã responses (p < 0.02) to influenza viruses in responders.
In conclusion, H1N1 vaccination induced substantial cell-mediated immunity, and to a lesser extent, humoral immunity at early times post-HSCT. H1N1/H3N2 T-cell cross-reactivity and protective (IL2) rather than effector (IFN¦Ã) cytokinic profiles were elicited.