Combination of Astragalus membranaceus and Euonymus alatus (THUNB.) SIEB. for treatment of diabetes: A network-based pharmacology research

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• 作者：Kun Chen^a ; ¹ ; Dong Deng^a ; ¹ ; Youliang Huang^a ; ¹ ; Xiaoyun Ma^a ; Xueli Li^a ; Wenting Zhang^a ; Zhiyong Li^b ; Yang Liu^a ; Wei Wang^a ; Jianxin Chen^a ; ^{cjx@bucm.edu.cn} ; HuiHui Zhao^a ; ^{zhaohh@bucm.edu.cn}
• 关键词：Astragalus ; Euonymus alatus ; Diabetes ; ESR1 ; REN
• 刊名：Journal of Traditional Chinese Medical Sciences
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：3
• 期：3
• 页码：184-191
• 全文大小：771 K
• 卷排序：3

文摘

To find the target genes in combination of astragalus root (Astragalus membranaceus) and spindle tree wings (Euonymus alatus (THUNB.) SIEB.) (Qijian Heji) with the function of regulating blood glucose levels in kk mice by real-time fluorescence quantitative PCR (RT-PCR). To achieve the primary exploration on the material basis for Qijian Heji to improve the blood glucose levels of type 2 diabetic kk mice.MethodsWe applied RT-PCR to detecting the expression of gene targets in kidney tissues of type 2 diabetes model kk mice. The detected gene targets were predicted by the network pharmacology method in the early stage of the project. Through the observation of the hypoglycemic effect of different ratios of Qijian Heji, the optimal proportion was determined and then used to conduct research of material basis.ResultsContinuous administration of Qijian Heji (astragalus root: spindle tree wings = 3:1) for six weeks resulted in significantly lowering blood glucose levels in mice in the fourth week, dropping from 33.3 to 21.9 mmol/L; this lower level was maintained in the fifth to sixth weeks. Blood glucose values in the positive drug group decreased from 33.3 to 21.85 mmol/L in the fourth week and maintained steady decreasing in the fifth and sixth weeks.ConclusionQijian Heji, with proportion of 3:1, could significantly reduce the blood glucose values in kk mice, animal experiment results verified the accurate prediction of network pharmacology, and at the same time, it is verified there was a certain therapeutic effect about the combination of the two herbs on reducing the glucose value. Animal experiments and RT-PCR results showed that Qijian Heji conducted a hypoglycemic effect by modulating estrogen receptor (ESR1) and renin (REN) expression.