Temporal biological variability in dendritic cells and regulatory T cells in peripheral blood of healthy adults
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- 作者：Maleewan Kitcharoensakkula ; 1 ; Leonard B. Bachariera ; 1 ; Huiqing Yin-Declueb ; 2 ; Jonathan S. Boomerb ; 2 ; Dana Burgdorfb ; 2 ; Brad Wilsonc ; 3 ; Kenneth Schechtmanc ; 3 ; Mario Castrob ; 2 ; castrom@dom.wustl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：DCs ; dendritic cells ; pDC ; plasmacytoid dendritic cells ; mDC1 ; myeloid dendritic cell subpopulation 1 ; mDC2 ; myeloid dendritic cell subpopulation 2 ; Tregs ; regulatory T cells ; nTreg ; natural Tregs ; iTreg ; CD3/CD46 induced Tregs ; IBV ; intra-individual biological variability ; PBMCs ; peripheral blood mononuclear cells ; FBS ; fetal bovine serum ; GZB ; granzyme B ; ICCs ; intraclass correlation coefficient
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：431
- 期：Complete
- 页码：63-65
- 全文大小：180 K
文摘
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There is substantial day-to-day variability in measuring mDC2 in vivo and iTreg production of IL-10 in vitro.
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There is a low intra-individual variability in measuring nTregs, pDCs and mDC1 in healthy adults.
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Repeat measurements over time of human biologic samples should be performed to determine the most representative values.