Critical assessment of three high performance liquid chromatography analytical methods for food carotenoid quantification

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• 作者：M. Graç ; a Dias ; Luí ; sa Oliveira ; M. Filomena G.F.C. Camõ ; es ; Baltazar Nunes ; Pieter Versloot ; Paul J.M. Hulshof
• 关键词：Carotenoids ; Food ; HPLC ; Method development ; Solvent and time reduction
• 刊名：Journal of Chromatography A
• 出版年：2010
• 出版时间：21 May 2010
• 年：2010
• 卷：1217
• 期：21
• 页码：3494-3502
• 全文大小：349 K

文摘

Three sets of extraction/saponification/HPLC conditions for food carotenoid quantification were technically and economically compared. Samples were analysed for carotenoids α-carotene, β-carotene, β-cryptoxanthin, lutein, lycopene, and zeaxanthin. All methods demonstrated good performance in the analysis of a composite food standard reference material for the analytes they are applicable to. Methods using two serial connected C₁₈ columns and a mobile phase based on acetonitrile, achieved a better carotenoid separation than the method using a mobile phase based on methanol and one C₁₈-column. Carotenoids from leafy green vegetable matrices appeared to be better extracted with a mixture of methanol and tetrahydrofuran than with tetrahydrofuran alone. Costs of carotenoid determination in foods were lower for the method with mobile phase based on methanol. However for some food matrices and in the case of E–Z isomer separations, this was not technically satisfactory. Food extraction with methanol and tetrahydrofuran with direct evaporation of these solvents, and saponification (when needed) using pyrogallol as antioxidant, combined with a HPLC system using a slight gradient mobile phase based on acetonitrile and a stationary phase composed by two serial connected C₁₈ columns was the most technically and economically favourable method.