Perilla frutescens leaves extract ameliorates ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin

详细信息    查看全文

• 作者：Jung-Soo Bae^a ; ^b ; ^c ; ¹ ; Mira Han^a ; ^b ; ^c ; ¹ ; Hee Soon Shin^d ; Min-Kyoung Kim^a ; ^b ; ^c ; Chang-Yup Shin^a ; ^b ; ^c ; Dong Hun Lee^a ; ^b ; ^c ; ^{ivymed27@snu.ac.kr} ; Jin Ho Chung^a ; ^b ; ^c ; ^e ; ^{jhchung@snu.ac.kr}
• 关键词：AP-1 ; activator protein-1 ; ECM ; extracellular matrix ; EMSA ; electrophoretic mobility shift assays ; ERK ; extracellular signal-regulated kinase ; HDF ; human dermal fibroblast ; JNK ; c-Jun N-terminal kinase ; MED ; minimal erythema dose ; MMP ; matrix metalloproteinases ; MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)&minus ; 2 ; 5-diphenyltetrazolium bromide ; PBS ; phosphate-buffered saline ; PLE ; Perilla frutescens leaves extract ; ROS ; reactive oxygen species ; SDS-PAGE ; sodium dodecyl sulfate-polyacrylamide gel electropho
• 刊名：Journal of Ethnopharmacology
• 出版年：2017
• 出版时间：4 January 2017
• 年：2017
• 卷：195
• 期：Complete
• 页码：334-342
• 全文大小：1433 K
• 卷排序：195

文摘

Perilla frutescens (L.) Britt. (Lamiaceae) is a traditional herb that is consumed in East Asian countries as a traditional medicine. This traditional herb has been documented for centuries to treat various diseases such as depression, allergies, inflammation and asthma. However, the effect of Perilla frutescens on skin has not been characterized well.Aim of the studyThe present study aimed to investigate the effect of Perilla frutescens leaves extract (PLE) on ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin.Materials and methodsHuman dermal fibroblasts and Skh-1 hairless mice were irradiated with UV and treated with PLE. Protein and mRNA levels of various target molecules were analyzed by western blotting and quantitative RT-PCR, respectively. Histological changes of mouse skin were analyzed by H&E staining. To elucidate underlying mechanism of PLE, activator protein-1 (AP-1) DNA binding assay and the measurement of reactive oxygen species (ROS) were performed.ResultsPLE significantly inhibited basal and UV-induced MMP-1 and MMP-3 expression dose-dependently, and also decreased UV-induced phosphorylation of extracellular signal-regulated kinases and c-Jun N-terminal kinases. This inhibitory effects of PLE on MMP-1 and MMP-3 were mediated by reduction of ROS generation and AP-1 DNA binding activity induced by UV. Furthermore, PLE promoted type I procollagen production irrespective of UV irradiation. In the UV-irradiated animal model, PLE significantly reduced epidermal skin thickness and MMP-13 expression induced by UV.ConclusionOur results demonstrate that PLE has the protective effect against UV-induced dermal matrix damage. Therefore, we suggest that PLE can be a potential agent for prevention of skin aging.