Human transbodies to VP40 inhibit cellular egress of Ebola virus-like particles
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- 作者：Salma Teimooria ; Watee Seesuaya ; Surasak Jittavisutthikula ; Urai Chaisrib ; Nitat Sookrungc ; Jaslan Densumitea ; d ; Nawannaporn Saelima ; Monrat Chulanetraa ; Santi Maneewatche ; Wanpen Chaicumpaa ; wanpen.cha@mahidol.ac.th" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cell penetrating antibody (transbody) ; Ebola virus ; Human scFv ; Scanning electron microscopy ; Virus-like particles ; VP40
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：14 October 2016
- 年：2016
- 卷：479
- 期：2
- 页码：245-252
- 全文大小：1841 K
文摘
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Cell penetrable human scFvs (transbodies) to Ebolavirus (EBOV) VP40 were produced.
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The transbodies inhibited egress of EBOV-like particles (VLPs) from human hepatocytes.
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They interacted with VP40 CTD basic residues important for plasma membrane binding.
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And hence interfere with viral matrix assembly and viral progeny budding.
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This is the first report on human antibodies that target intracellular EBOV VP40.