Crystallization and preliminary X-ray crystallographic study of the leech protease inhibitor guamerin and its complex with bovine pancreatic chymotrypsin

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• 作者：Kim ; Dong Ryoung ; Kim ; Dong Young ; Chu ; Trang Thi Thu ; Jung ; Kyung-Hwan ; Kim ; Kyeong Kyu
• 关键词：Guamerin ; Chymotrypsin ; Crystallization
• 刊名：Biochimica et Biophysica Acta - Proteins and Proteomics
• 出版年：2004
• 出版时间：June 1, 2004
• 年：2004
• 卷：1699
• 期：1-2
• 页码：285-287
• 全文大小：98.8 K

文摘

Guamerin, a small peptide inhibitor of the serine protease from Hirudo nipponia, was expressed in yeast and crystallized using the vapor diffusion method, with MPD as precipitant. The crystal was found to belong to the monoclinic P2₁ space group with unit cell parameters a