Molecular cloning and expression analysis of two lipopolysaccharide-induced TNF-伪 factors (LITAFs) from rock bream, Oplegnathus fasciatus

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• 作者：Seong Don Hwang ; Sang Hee Shim ; Mun-Gyeong Kwon ; Young Sun Chae ; Won Joon Shim ; Jee-Hyun Jung ; Ju-Won Kim ; Chan-Il Park
• 关键词：LITAF ; Oplegnathus fasciatus ; Edwardsiella tarda ; Streptococcus iniae ; Red seabream iridovirus
• 刊名：Fish & Shellfish Immunology
• 出版年：February, 2014
• 年：2014
• 卷：36
• 期：2
• 页码：467-474
• 全文大小：1700 K

文摘

Lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-伪 factor (LITAF) plays an important role controlling the expression of TNF-伪 and the other cytokine genes in the presence of LPS. However, two LITAF homologues have not been characterized in fish. In this study, we cloned two distinct LITAF (RbLITAF1 and RbLITAF2) cDNAs from rock bream (Oplegnathus fasciatus) and characterized their expression profiles after infection with Edwardsiella tarda, Streptococcus iniae or red seabream iridovirus (RSIV). The coding regions of RbLITAF1 and RbLITAF2 cDNAs were 492聽bp and 417聽bp, encoding 153 and 138 amino acid residues, respectively. The genes consisted of a LITAF domain. RbLITAF1 was highly expressed in the spleen and heart of healthy rock bream, whereas RbLITAF2 was highly expressed in the gill, intestine and stomach. In spleen, the gene expression of RbLITAF1 and RbLITAF2 were increased until 5 days post-infection (dpi), and then decreased at 7聽dpi. In kidney, E.聽tarda and RSIV infection led to induction of the RbLITAF1 gene at 1聽dpi, RbLITAF2 gene was down-regulated after pathogen infection. These results suggest that RbLITAFs may be involved in the LITAF-mediated immune response and regulate systemic immune responses against pathogen infection.