Production of specific-molecular-weight hyaluronan by metabolically engineered Bacillus subtilis 168
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- 作者：Peng Jina ; Zhen Kanga ; b ; c ; zkang@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Panhong Yuana ; Guocheng Dua ; b ; c ; Jian Chena ; b ; c ; d ; jchen@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hyaluronan ; Low molecular weight ; Oligosaccharide ; Hyaluronidase ; Metabolic engineering ; Bacillus subtilis
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：35
- 期：Complete
- 页码：21-30
- 全文大小：1515 K
文摘
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Biosynthetic pathways for GlcUA and GlcNAc were optimized in Bacillus subtilis.
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Leech hyaluronidase (LHAase) was expressed and secreted to 1.62×106 U mL−1.
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Hyaluronan titer increased significantly (19.38 g L−1) with hyaluronidase expression.
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Low-molecular-weight hyaluronans were produced by controllable expression of LHAase.