Enzymatically cross-linked alginic-hyaluronic acid composite hydrogels as cell delivery vehicles

详细信息    查看全文

• 作者：Nitya Ganesh^a ; ¹ ; Craig Hanna^b ; ¹ ; Shantikumar V. Nair^a ; ^{shantinair@aims.amrita.edu} ; Lakshmi S. Nair^b ; ^c ; ^{nair@uchc.edu}
• 关键词：Alginic acid ; Hyaluronic acid ; Enzymatic cross-linking ; Composite gels
• 刊名：International Journal of Biological Macromolecules
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：55
• 期：Complete
• 页码：289-294
• 全文大小：723 K

文摘

An injectable composite gel was developed from alginic and hyaluronic acid. The enzymatically cross-linked injectable gels were prepared via the oxidative coupling of tyramine modified sodium algiante and sodium hyaluronate in the presence of horse radish peroxidase (HRP) and hydrogen peroxide (H₂O₂). The composite gels were prepared by mixing equal parts of the two tyraminated polymer solutions in 10 U HRP and treating with 1.0 % H₂O₂. The properties of the alginate gels were significantly affected by the addition of hyaluronic acid. The percentage water absorption and storage modulus of the composite gels were found to be lower than the alginate gels. The alginate and composite gels showed lower protein release compared to hyaluronate gels in the absence of hyaluronidase. Even hyaluronate gels showed only approximately 10 % protein release after 14 days incubation in phosphate buffer solution. ATDC-5 cells encapsulated in the injectable gels showed high cell viability. The composite gels showed the presence of enlarged spherical cells with significantly higher metabolic activity compared to cells in hyaluronic and alginic acid gels. The results suggest the potential of the composite approach to develop covalently cross-linked hydrogels with tuneable physical, mechanical, and biological properties.