Application of an efficient and easy method for the introduction of additional disulfide bonds without the need of a crystal structure.
CHMO variants created with higher thermal as well as oxidative stability without any loss of catalytic activity.
Additional creation and analysis of single Cys/Ser mutants.
20">Major improvement of the enzyme half-life by only one exceptional cysteine mutation.
25">ABD-F labeling as fast assay to verify the correct formation of disulfide bonds.