- 作者：Fatima Saqib ; Fatima2saqib@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Fatima_pharmacology@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Khalid Hussain Janbaz khjanbaz@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Carbachol/Carbamylcholine chloride (CID ; 5831) ; Verapamil Hydrochloride (CID ; 2520) ; Phenylephrine (CID ; 6041) ; Ketamine (CID ; 3821) ; Diazepam (CID ; 3061) ; Acetylcholine chloride (CID ; 6060) ; Adrenaline (CID ; 5816)
- 刊名：Journal of Ethnopharmacology
- 出版年：2016
- 出版时间：22 April 2016
- 年：2016
- 卷：182
- 期：Complete
- 页码：110-121
- 全文大小：2651 K
Objective
The current study was conducted to provide mechanistic basis for anti-spasmodic, anti- asthmatic and anti-hypertensive use of Alternanthera sessilis.
Materials and methods
The crude ethanolic extract of Alternanthera sessilis (As.Cr) and its fractions were tested in- vitro on isolated rabbit tissue preparations (i.e., jejunum, trachea, and aorta) and in-vivo in ketamin-diazepam anaesthetized normotensive rats.The responses were recorded using isotonic and isometric transducers coupled with Power Lab data acquisition system.
Results
On isolated rabbit jejunum preparations, As.Cr exerted concentration-dependent (0.01–1.0 mg/ml) spasmolytic effect and caused relaxation of K+(80 mM)-induced spastic contractions. Furthermore, there was non-parallel shift in Ca++concentration response curves (CRCs) towards right at tissue bath concentrations of 0.1 and 0.3 mg/ml. On isolated rabbit trachea, it relaxed carbachol (1 μM)- and K+(80 mM)-induced contractions at respective tissue bath concentrations of 5.0 and 1.0 mg/ml. On isolated rabbit aorta, it also demonstrated relaxant effect on phenylephrine (1 μM)- and K+(80 mM)-induced contractions at tissue bath concentrations of 5.0 and 3.0 mg/ml respectively. These findings were found to be comparable with verapamil, a reference Ca++channel blocker (CCB). The solvent-solvents fractionation revealed domination of spasmolytic effects in dichloromethane fraction as compared to aqueous fraction. Intravenous administration of As.Cr decreased mean arterial blood pressure, systolic blood pressure and diastolic blood pressure of ketamine-diazepam anaesthetized normotensive albino rats dose-dependently, at dose range of 1–10 mg/kg.
Conclusion
Our results reflected presence of Ca++ channel blocking (CCB) activity in As.Cr, thus rationalizing medicinal use of Alternanthera sessilis in diarrhea, asthma and hypertension.