Synthesis and characterization of nanosilver-silicone hydrogel composites for inhibition of bacteria growth

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• 作者：F.M. Helaly^a ; S.M. El-Sawy^a ; A.I. Hashem^b ; A.A. Khattab^c ; R.M. Mourad^a ; ^{rmmay80@yahoo.com}
• 关键词：Silicone hydrogel ; Contact lenses ; Silver nanoparticles ; Antimicrobial
• 刊名：Contact Lens and Anterior Eye
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：40
• 期：1
• 页码：59-66
• 全文大小：2111 K
• 卷排序：40

文摘

Nanosilver-silicone hydrogel (NAgSiH) composites for contact lenses were synthesized to asses the antimicrobial effects.MethodsSilicone hydrogel (SiH) films were synthesized followed by impregnation in silver nitrate solutions (10, 20, 30, 40, 60, 80 ppm) and in-situ chemical reduction of silver ions using sodium borohydride (NaBH4). The silver nano particles (AgNPS) were identified by UV–vis absorption spectroscopy, Energy-dispersive X-ray spectroscopy (EDX) mapping and EDX spectrum. Physico-mechanical and chemical properties of NAgSIH films were studied. The antimicrobial effect of the hydrogels against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus was evaluated. The numbers of viable bacterial cells on NAgSiH surface or in solution compared to control SiH were examined.ResultsThe NAgSiH films were successfully synthesized. FTIR results indicated that AgNPS had no effect on the bulk structure of the prepared SiH films. From TGA analysis, NAgSiH(R80) and SiH(R0) films had the same maximum decomposition temperature (404 °C). UV–vis absorption spectroscopy and EDX mapping and spectrum emphasized that AgNPS were in spherical shape. The maximum absorption wavelength of NAgSiH films were around 400 nm. The light transmittance decreased as the concentration of AgNPS increased, but still greater than 90% at wavelength around 555 nm. The Young’s modulus increased gradually from 1.06 MPa of SiH(R0) to highest value 1.38 MPa of NAgSiH(R80). AgNPS incorporated into SiH films reduced the bacterial cell growth and prevented colonization. Groups NAgSiH(R60,R80) demonstrated an excellent reduction in bacterial viability in solution and on the SiH surface.ConclusionsNAgSiH composites were successfully synthesized and possessed an excellent antimicrobial effects.