Spatial quantification of hydrogels swelling using wide-field fluorescence microscopy

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• 作者：Weiji Liu^a ; Xiao Dong Chen^a ; ^b ; ^{xdcxmu@gmail.com} ; Ruben Mercadé ; -Prieto^b ; ^{ruben@suda.edu.cn}
• 关键词：Whey protein hydrogels ; Swelling ; Fluorescence ; Rhodamine B
• 刊名：Chemical Engineering Science
• 出版年：2017
• 出版时间：2 February 2017
• 年：2017
• 卷：158
• 期：Complete
• 页码：349-358
• 全文大小：2084 K
• 卷排序：158

文摘

Simple technique is required to measure the local swelling degree of hydrogels. Whey protein hydrogels are made stained with Rhodamine B fluorescent dye. Wide-field fluorescence microscopy can quantify the total fluorescence in thick transparent gels. Median fluorescence in hydrogels decreases during swelling as expected in isotropic swelling. 2D spatial information of the dynamic swelling is obtained for the first time in protein hydrogels.