Combination of enzymatic hydrolysis and ethanol organosolv pretreatments: Effect on lignin structures, delignification yields and cellulose-to-glucose conversion

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• 作者：Patrick Obama^a ; Guillaume Ricochon^b ; Lionel Muniglia^b ; Nicolas Brosse^a ; ^{Nicolas.Brosse@lermab.uhp-nancy.fr}
• 关键词：Enzyme ; Miscanthus ; Pretreatment ; Bioethanol
• 刊名：Bioresource Technology
• 出版年：2012
• 出版时间：May, 2012
• 年：2012
• 卷：112
• 期：Complete
• 页码：156-163
• 全文大小：558 K

文摘

Enzymatic pre-hydrolysis using the industrial enzymatic cocktail Cellulyve? was assessed as a first step in a pretreatment process of Miscanthus biomass involving an aqueous-ethanol organosolv treatment. ¹³C and ³¹P Nuclear Magnetic Resonance and size exclusion chromatography were used to analyze the cellulose and lignin before and after treatment. It was demonstrated that despite a very low impact on the fibre structure (observed by Scanning Electron Microscopy) and composition (in terms of sugars and polyphenolics content), the enzymatic pre-treatment disrupted the lignocellulosic matrix to a considerable extend. This weakening permitted enhanced removal of lignin during organosolv pulping and increased hydrolysability of the residual cellulosic pulp for the production of monomeric glucose. Using this combined treatment, a delignification yield of 93 % and an enzymatic cellulose-to-glucose conversion of 75 % were obtained.