Effect of enzyme load and catalyst particle size on the diffusional restrictions in reactions of synthesis and hydrolysis catalyzed by α-chymotrypsin immobilized into glyoxal-agarose

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• 作者：Carola Bahamondes^a ; ^{carolasbd@yahoo.com} ; Gregorio Á ; lvaro^b ; Lorena Wilson^a ; André ; s Illanes^a
• 关键词：Peptide synthesis ; α-chymotrypsin ; Kyotorphin ; Diffusional restrictions ; Heterogeneous biocatalysis
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：53
• 期：Complete
• 页码：172-179
• 全文大小：690 K
• 卷排序：53

文摘

α-Chymotrypsin was immobilized in glyoxal agarose. Hydrolysis and synthesis reactions were modelled using Michaelis-Menten kinetics. Km and Vm were determined on different support sizes and enzyme loads. Internal diffusional restrictions were evaluated in hydrolysis and synthesis. Maximum size and load to avoid diffusional restrictions were determined.