Development of an injectable PHBV microparticles-GG hydrogel hybrid system for regenerative medicine

详细信息    查看全文

• 作者：Daniela P. Pacheco^a ; ^b ; ^c ; ; Maria H. Amaral^c ; Rui L. Reis^a ; ^b ; Alexandra P. Marques^a ; ^b ; Ví ; tor M. Correlo^a ; ^b ; ^{vitorcorrelo@dep.uminho.pt" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Polyhydroxybutyrate-co-hydroxyvalerate ; Gellan gum ; Microparticulate systems ; Injectable hydrogel ; Regenerative medicine
• 刊名：International Journal of Pharmaceutics
• 出版年：2015
• 出版时间：15 January 2015
• 年：2015
• 卷：478
• 期：1
• 页码：398-408
• 全文大小：2285 K

文摘

Uncontrollable displacements that greatly affect the concentration of active agents at the target tissues are among a major limitation of the use of microparticulate drug delivery systems (DDS). Under this context a biphasic injectable DDS combining poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles (MPs) and a gellan gum (GG) injectable hydrogel is herein proposed for the localized delivery and long-term retention of MPs carrying hydrophilic and hydrophobic model active agents. A double emulsion-solvent evaporation method was adopted to develop the PHBV MPs, carrying bovine serum albumin (BSA) or dexamethasone (Dex) as hydrophilic and hydrophobic active agents’ models, respectively. Moreover, this method was modified, together with the properties of the hydrogel to tailor the delivery profile of the active agents. Variations of the composition of the organic phase during the process allowed tuning surface topography, particle size distribution and core porosity of the PHBV MPs and, thus, the in vitro release profile of Dex but not of BSA. Besides, after embedding hydrogels of higher GG concentration led to a slower and more sustained release of both active agents, independently of the processing conditions of the microparticulate system.