Lotus japonicus plants of the Gifu B-129 ecotype subjected to alkaline stress improve their Fe²⁺ bio-availability through inoculation with Pantoea eucalypti M91

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• 作者：Marí ; a Paula Campestre^a ; ¹ ; ^{mpcampestre@intech.gov.ar" class="auth_mail" title="E-mail the corresponding author} ; Luis Nazareno Castagno^b ; ¹ ; ^{ncastagno@intech.gov.ar" class="auth_mail" title="E-mail the corresponding author} ; Marí ; a Julia Estrella^b ; ² ; ^{estrella@intech.gov.ar" class="auth_mail" title="E-mail the corresponding author} ; Oscar Adolfo Ruiz^a ; ^{ruiz@intech.gov.ar" class="auth_mail" title="E-mail the corresponding author} ; ^{oaruiz@conicet.gov.ar" class="auth_mail" title="E-mail the corresponding author}
• 关键词：PSII ; photosystem II ; FRO ; ferric reductase oxidase ; IRT ; iron regulated transporter ; PGPB ; plant growth promoting bacteria ; CAS ; chrome-azurol S ; RFP ; red fluorescent protein ; DW ; dry weight ; PI ; performance index ; TT ; topological tendency ; RU ; relative units ; ANOVA ; analysis of variance ; VOC&rsquo ; s ; volatile organic compounds ; IAA ; indole-3-acetic acid
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：15 March 2016
• 年：2016
• 卷：192
• 期：Complete
• 页码：47-55
• 全文大小：2066 K

文摘

Inoculation assays with Pantoea eucalypti M91 were performed on Lotus japonicus ecotype Gifu. Under alkaline conditions, this ecotype is characterized by the development of interveinal chlorosis of the apical leaves due to low mobilization of Fe²⁺. Inoculation with P. eucalypti M91, a plant growth-promoting bacterial strain capable of producing pyoverdine-like and pyochelin-like siderophores under alkaline growth conditions, alters the root, resulting in a herringbone pattern of root branching. Additional features include improvement in Fe²⁺ transport to the shoots, acidification of the hydroponic solution of the plant cultures, and an accompanying increase in the efficiency of the PSII parameters. In addition, there was an increase in the expression of the FRO1 and IRT1 genes, accompanied by a significant increase in FRO activity. Results showed that P. eucalypti M91 has a beneficial effect on the Fe acquisition machinery of Strategy I, as described for non-graminaceous monocots and dicots, suggesting its potential as an inoculant for legume crops cultivated in alkaline soils.