De novo biosynthesis of Gastrodin in Escherichia coli
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- 作者：Yanfen Baia ; b ; c ; Hua Yina ; b ; Huiping Bia ; b ; Yibin Zhuanga ; b ; Tao Liua ; b ; liu_t@tib.cas.cn" class="auth_mail" title="E-mail the corresponding author ; Yanhe Maa
- 关键词：Gastrodin (PubChem CID ; 115067) ; 4-Hydroxybenzyl alcohol (PubChem CID ; 125) ; Tyrosol (PubChem CID ; 10393) ; Salidroside (PubChem CID ; 159278) ; 4-Hydroxybenzoic acid (PubChem CID ; 135) ; 4-Methylumbelliferone (PubChem CID ; 5280567) ; 4-Methylumbelliferyl β-D-glucoside (PubChem CID ; 2733779) ; 4-Hydroxybenzoic acid β-D-glucopyranoside (PubChem CID ; 440186) ; 4-Formylphenyl β-D-glucopyranoside (PubChem CID ; 7573800)
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：35
- 期：Complete
- 页码：138-147
- 全文大小：1680 K
文摘
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An artificial novel biosynthetic pathway for gastrodin was constructed.
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Gastrodin was produced from glucose in an E. coli factory for the first time.
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Pathway genes were overexpressed to enhance metabolic flux toward aglycon.
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Directed evolution was applied to improve catalytic properties of UGT73B6.
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∽545 mg/L of gastrodin was produced by the finally engineered E. coli.