Development and validation of a high-throughput stereoselective LC-MS/MS assay for bupropion, hydroxybupropion, erythrohydrobupropion, and threohydrobupropion in human plasma
详细信息 查看全文
- 作者：Aaron M. Teitelbauma ; Alicia M. Flakera ; Evan D. Kharascha ; b ; c ; kharasch@wustl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bupropion ; Hydroxybupropion ; Erythrohydrobupropion ; Threohydrobupropion ; LC&ndash ; MS/MS ; Stereoselectivity
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：1017-1018
- 期：Complete
- 页码：101-113
- 全文大小：2696 K
文摘
- •
A stereoselective analytical method for bupropion and metabolites was developed and validated on three triple quadrupole mass spectrometers.
- •
Bupropion, hydroxybupropion, erythrohydrobupropion, and threohydrobupropion enantiomers were resolved with a α1-acid glycoprotein chiral column.
- •
Erythrohydrobupropion and threohydrobupropion enantiomer peaks were stereochemically assigned by borohydride reduction of enantiopure (R and S)-bupropion.
- •
Plasma concentrations of bupropion and metabolite isomers were determined from a patient who received extended release (R,S)-bupropion.