- 作者：Aaron M. Teitelbauma ; Alicia M. Flakera ; Evan D. Kharascha ; b ; c ; kharasch@wustl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bupropion ; Urine ; LC&ndash ; MS/MS ; Analytical method ; Stereoselectivity
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 August 2016
- 年：2016
- 卷：1027
- 期：Complete
- 页码：239-253
- 全文大小：2708 K
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A stereoselective assay for bupropion and oxidative, reductive, and glucuronide metabolites (16 analytes total) in human urine was developed and validated.
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A trifurcated sample preparation and analysis procedure was used for chiral analysis of (a) free bupropion and primary metabolites, (b) total hydroxybupropion after hydrolysis, and (c) glucuronide metabolites.
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Erythrohydrobupropion and threohydrobupropion glucuronides underwent diastereomer-specific hydrolysis by β-glucuronidase.
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Stereochemistry of glucuronide standards obtained from commercial sources was found to be incorrectly labeled, and the correct stereochemistry was unequivocally reassigned.
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Urine concentration of bupropion and metabolite isomers were determined from a patient who received extended release (R,S)-bupropion.