Rapid LC-MS/MS method for the determination of 4-hydroxycholesterol/cholesterol ratio in serum as endogenous biomarker for CYP3A activity in human and foals
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- 作者：Mahmoud Hasan1 ; Werner Siegmund ; Stefan Oswald ; stefan.oswald@uni-greifswald.de" class="auth_mail" title="E-mail the corresponding author (Prof. ; Dr.)
- 关键词：APCI ; atmospheric pressure chemical ionization ; CHO ; cholesterol ; HO-CHO ; hydroxycholesterol ; d6-CHO ; 6-deuterated cholesterol ; d7-4-HO-CHO ; 7-deuterated 4β-hydroxycholesterol ; LC&ndash ; MS/MS ; liquid chromatography tandem mass spectrometry ; GC/MS ; gas chromatography coupled with mass spectrometry ; LLOQ ; lower limit of quantification ; MRM ; multiple reaction monitoring ; HSA ; human serum albumin ; DDI ; clinical drug&ndash ; drug interaction
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：1033-1034
- 期：Complete
- 页码：193-199
- 全文大小：976 K
文摘
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Simultaneous quantification of cholesterol and its CYP3A4 dependent metabolite 4ß-OH-cholesterol with one analytical method (LC–MS/MS).
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No further derivatization needed; i.e. direct determination (established is so far the formation of piconyl esters).
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Similar or even higher sensitivity compared to previously published methods and considerably faster chromatographic run time than available methods (8 min vs. 10–30 min).
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20">Comprehensive method validation according to current bioanalytical guidelines.
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Application of the method to monitor induction effects of rifampicin in human and foals.