Fed-batch production of poly-3-hydroxydecanoate from decanoic acid
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- 作者：Jie Gaoa ; Juliana A. Ramsaya ; Bruce A. Ramsaya ; b ; Bruce.Ramsay@Polyferm.com" class="auth_mail" title="E-mail the corresponding author ; Bruce.Ramsay@queensu.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Medium-chain-length poly-3-hydroxyalkanoate ; MCL-PHA ; Decanoic acid ; Fed-batch ; Fermentation ; Pseudomonas putida
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 January 2016
- 年：2016
- 卷：218
- 期：Complete
- 页码：102-107
- 全文大小：790 K
文摘
- 5">
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5">Decanoic acid is an ideal substrate for the synthesis of MCL-PHA, but its use for this purpose had only been studied in shake-flasks.
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A fed-batch fermentation process was developed for the production of MCL-PHA from decanoic acid using Pseudomonas putida KT2440.
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5">Decanoic acid was kept in liquid form by heating or by mixing with acetic acid.
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Different ratios of decanoic acid:acetic acid:glucose (DA:AA:G) were fed.
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5">75 g L−1 biomass containing 74% PHA were produced with each gram of PHA requiring only 1.16 g of decanoic acid.