Inhibition of 3β- and 17β-hydroxysteroid dehydrogenase activities in rat Leydig cells by perfluorooctane acid

详细信息    查看全文

• 作者：Binghai Zhao ; Yanhui Chu ; Dianne O. Hardy ; Xiao-kun Li ; Ren-Shan Ge
• 关键词：PFOA ; Enzyme inhibition ; 3&#x3b2 ; -Hydroxysteroid dehydrogenase ; 17&#x3b2 ; -Hydroxysteroid dehydrogenase 3
• 刊名：The Journal of Steroid Biochemistry and Molecular Biology
• 出版年：2010
• 出版时间：January 2010
• 年：2010
• 卷：118
• 期：1-2
• 页码：13-17
• 全文大小：459 K

文摘

Perfluorooctane acid (PFOA) is classified as a persistent organic pollutant and as an endocrine disruptor. The mechanism by which PFOA causes reduced testosterone production in males is not known. We tested our hypothesis that PFOA interferes with Leydig cell steroidogenic enzymes by measuring its effect on 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-hydroxysteroid dehydrogenase 3 (17β-HSD3) activities in rat testis microsomes and Leydig cells. The IC₅₀s of PFOA and mode of inhibition were assayed. PFOA inhibited microsomal 3β-HSD with an IC₅₀ of 53.2 ± 25.9 μM and 17β-HSD3 with an IC₅₀ 17.7 ± 6.8 μM. PFOA inhibited intact Leydig cell 3β-HSD with an IC₅₀ of 146.1 ± 0.9 μM and 17β-HSD3 with an IC₅₀ of 194.8 ± 1.0 μM. The inhibitions of 3β-HSD and 17β-HSD3 by PFOA were competitive for the substrates. In conclusion, PFOA inhibits 3β-HSD and 17β-HSD3 in rat Leydig cells.