Functionalized mesoporous silicon for targeted-drug-delivery

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• 作者：Ozra Tabasi ; Cavus Falamaki ; Zahra Khalaj
• 关键词：Targeted drug delivery ; Porous silicon ; Doxorubicin ; Folate ; Cytotoxicity
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2012
• 出版时间：1 October, 2012
• 年：2012
• 卷：98
• 期：Complete
• 页码：18-25
• 全文大小：920 K

文摘

The present work concerns a preliminary step in the production of anticancer drug loaded porous silicon (PSi) for targeted-drug-delivery applications. A successful procedure for the covalent attachment of folic acid, polyethylene glycol (PEG) and doxorubicin to hydrophilic mesoporous silicon layers is presented. A systematic approach has been followed to obtain the optimal composition of the N,N¡ä-dicyclohexylcarbodiimide (DCC)/N-hydroxysuccimide (NHS) in dimethylsulfoxide (DMSO) solution for the surface activation process of the undecylenic acid (UD) grafted molecules to take place with minimal undesired byproduct formation. The effect of reactant concentration and kind of solvent (aqueous or DMSO) on the attachment of folic acid to the activated PSi layer has been investigated. The covalent attachment of the doxorubicin molecules to the PSi layer functionalized with folic acid and PEG is discussed. The drug release kinetics as a function of pH has been studied. The functionalized PSi particles show a high cytotoxicity compared to the equivalent amount of free drug. Cell toxicity tests show clearly that the incorporation of folate molecules increases substantially the toxicity of the loaded PSi particles. Accordingly this new functionalized PSi may be considered a proper candidate for targeted drug delivery.