In silico analysis of the effects of H₂ and CO₂ on the metabolism of a capnophilic bacterium Mannheimia succiniciproducens

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• 作者：Tae Yong Kim ; Hyun Uk Kim ; Hyohak Song ; Sang Yup Lee
• 关键词：Mannheimia succiniciproducens ; H₂ ; CO₂ ; Constraints-based flux analysis ; Succinic acid
• 刊名：Journal of Biotechnology
• 出版年：2009
• 出版时间：November 2009
• 年：2009
• 卷：144
• 期：3
• 页码：184-189
• 全文大小：359 K

文摘

This study presents an in-depth study on the physiological behavior of Mannheimia succiniciproducens, a capnophilic bacterium and an efficient succinic acid producer, under varying gas conditions as H₂ and CO₂ play important roles in the production of succinic acid. Constraints-based flux analysis of the genome-scale metabolic model of M. succiniciproducens was performed to estimate the production patterns of several organic acids in response to varying H₂, CO₂, and glucose uptake rates. Results from controlled cultivations performed previously and constraints-based flux analyses of M. succiniciproducens in this study revealed that there is an optimal range of CO₂ level in the medium for enhancing cell growth and succinic acid production at a given glucose uptake rate. Furthermore, the uptake rates of H₂ and CO₂ from the medium have a direct relationship with each other, significantly influencing the rates of cell growth and succinic acid production. Predictions made in this study quantitatively describe the physiological changes of the cell in response to varying H₂, CO₂, and glucose uptake rates, which consequently allow us to identify the feasible physiological states of the cell with respect to cell growth rate and succinic acid production rate.