(Z)-ligustilide increases ferroportin1 expression and ferritin content in ischemic SH-SY5Y cells

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• 作者：Yun-Ting Zhang^a ; ¹ ; Fei-Mi Li^a ; ^b ; ¹ ; Yi-Zhen Guo^a ; Li-Rong Jiang^a ; Juan Ma^a ; ^b ; Ya Ke^b ; ^{yake@cuhk.edu.hk} ; Zhong-Ming Qian^a ; ^{qianzhongming@fudan.edu.cn}
• 关键词：(Z)-ligustilide (LIG) ; Antioxidant and anti-apoptosis ; Iron metabolism proteins ; Oxygen-glucose deprivation/reoxygen (OGD/R) ; Hypoxia inducible factor-1 alpha (HIF-1 alpha) ; SH-SY5Y cells
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：5 December 2016
• 年：2016
• 卷：792
• 期：Complete
• 页码：48-53
• 全文大小：755 K
• 卷排序：792

文摘

The mechanisms involved in the antioxidant and anti-apoptotic properties of (Z)-ligustilide (LIG) are not fully elucidated. Based on the accumulated data, we hypothesized that LIG might be able to reduce ischemia/reperfusion-induced increase in brain iron by regulating expression of iron transport proteins. We therefore investigated the effects of LIG on iron uptake protein transferrin receptor 1, iron exporter protein ferroportin 1, iron storage protein ferritin light chain and also hypoxia inducible factor-1 alpha (HIF-1 alpha) in oxygen-glucose deprivation/reoxygenation (OGD/R)-treated SH-SY5Y cells, using Western blot analysis. We demonstrated that LIG completely reversed the OGD/R-induced reduction of ferroportin 1, increased ferritin light chain content, and also suppressed the OGD-induced increase in HIF-1 alpha in SH-SY5Y cells. These findings imply that LIG might reduce the OGD/R-induced increase in brain iron by promoting cell iron release and iron corporation into ferritin, and also by inhibiting the HIF-1 alpha-induced increase in transferrin-bound iron uptake and iron accumulation in the brain, consequently attenuating iron-mediated free radical formation, oxidative stress and apoptosis.