Extraction and fractionation of glucosyltransferase inhibitors from cacao bean husk

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• 作者：Kyoung Heon Kim ; Ki Won Lee ; Dong Young Kim ; Hyung Hwan Park ; Ik Boo Kwon ; Hyong Joo Lee
• 关键词：Cacao bean husk ; Theobroma cacao ; Glucosyltransferase ; Anticaries ; Polyphenols
• 刊名：Process Biochemistry
• 出版年：2004
• 出版时间：29 October 2004
• 年：2004
• 卷：39
• 期：12
• 页码：2043-2046
• 全文大小：61 K

文摘

The re-utilization of cacao bean husk, a waste generated from the chocolate industry, would bring benefits both environmentaly and economicaly. This study relates to a process for effectively separating and fractionating a cacao bean husk fraction having high inhibitory activity against glucosyltransferase (GTF) for the prevention of tooth decay (anticaries activity). Since the GTF inhibitory activity is known to be rendered by polyphenols, the separation process was also able to aim at high recovery of polyphenols which benefits human health. In this study, cacao bean husk extract, obtained under optimal conditions, extraction with 50 % (v/v) aq. (aqueous) acetone solution at 60 °C for 4 h followed by 50 % (v/v) aq. ethanol using a styrene-based resin, showed significantly higher inhibitory activity (2 and 12 folds, respectively) against GTF and a similar polyphenol content, compared to two other commercial anti-GTF polyphenol products.