In situ immobilization of lysine decarboxylase on a biopolymer by fusion with phasin: Immobilization of CadA on intracellular PHA
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- 作者：Hyung-Min Seoa ; 1 ; Jung-Ho Kima ; 1 ; Jong-Min Jeona ; Hun-Suk Songa ; Shashi Kant Bhatiaa ; b ; Ganesan Sathiyanarayanana ; Kyungmoon Parkc ; Kwang Jin Kimd ; Sang Hyun Leea ; Hyung Joo Kima ; Yung-Hun Yanga ; b ; seokor@konkuk.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cadaverine (PubChem CID ; 273) ; l-Lysine (PubChem CID ; 5962) ; (R)-3-Hydroxybutyric acid (PubChem CID ; 92135)
- 刊名：Process Biochemistry
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：51
- 期：10
- 页码：1413-1419
- 全文大小：1029 K
文摘
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An in situ immobilization system for enzymatic cadaverine production was developed.
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CadA was immobilized on poly (3-hydroxybutyrate) (P(3HB)) biopolymer granules.
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Increased P(3HB) production was observed with phasin-fused CadA.
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The thermal stability increased with immobilization.
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Repetitive reactions of the CadA–P(3HB) complex were observed.