Novel 3D printed alginate-BFP1 hybrid scaffolds for enhanced bone regeneration

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• 作者：Eun Young Heo^a ; ¹ ; Na Re Ko^a ; ¹ ; Min Soo Bae^a ; Sang Jin Lee^a ; Byung-Joon Choi^b ; Jung Ho Kim^a ; Hyung Keun Kim^c ; Su A Park^d ; ^{psa@kimm.re.kr} ; Il Keun Kwon^a ; ^{kwoni@khu.ac.kr}
• 关键词：Tissue engineering ; Hydrogel scaffold ; BFP1 ; 3D printing ; Bone regeneration
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2017
• 出版时间：25 January 2017
• 年：2017
• 卷：45
• 期：Complete
• 页码：61-67
• 全文大小：2303 K
• 卷排序：45

文摘

In this research, novel 3D printed alginate–peptide hybrid scaffolds were explored for enhanced bone regeneration. Bone formation peptide-1 (BFP1) was loaded into the scaffolds to promote bone regeneration. The alginate–BFP1 conjugates were synthesized via a facile EDC/NHS coupling reaction and then cross-linked in the presence of calcium ions to form hydrogel networks. The dose-dependent cytotoxicity of CaCl2 was evaluated to optimize the amount of Ca2+ for fabrication of scaffolds. In vitro and in vivo studies indicated that the alginate-based scaffolds provided a stable environment for the growth of human adipose-derived stem cells (hADSCs) and led to a synergistically enhanced bone regeneration.