Characterization of Lactobacillus fermentum UCO-979C, a probiotic strain with a potent anti-Helicobacter pylori activity

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• 作者：Apolinaria Garc&iacute ; a^a ; ^{apgarcia@udec.cl} ; Karen Navarro^a ; Enrique Sanhueza^a ; Susana Pineda^a ; Edgar Pastene^b ; Manuel Quezada^c ; Karem Henr&iacute ; quez^b ; Andrey Karlyshev^d ; Julio Villena^e ; Carlos Gonzá ; lez^a
• 关键词：Efficient colonization ; Gastric cancer ; Highly adherent to glass ; Hydrophobic bacteria ; Probiotic protection against gastric cancer ; Probiotic protection against Helicobacter pylori
• 刊名：Electronic Journal of Biotechnology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：25
• 期：Complete
• 页码：75-83
• 全文大小：638 K
• 卷排序：25

文摘

Helicobacter pylori is considered as the main risk factor in the development of gastric cancer. In the present study, we performed a detailed characterization of the probiotic properties and the anti-H. pylori activity of a previously isolated lactobacillus strain — Lactobacillus fermentum UCO-979C — obtained from human gut.ResultsThe strain tolerated pH 3.0; grew in the presence of 2% bile salts; produced lactic acid and hydrogen peroxide; aggregated in saline solution; showed high hydrophobicity; showed high adherence to glass; Caco-2 and gastric adenocarcinoma human cells (AGS) cells; showed an efficient colonization in Mongolian Gerbils; and potently inhibited the growth and urease activity of H. pylori strains. L. fermentum UCO-979C significantly inhibited H. pylori-induced IL-8 production in AGS cells and reduced the viability of H. pylori. With regard to innocuousness, the strain UCO-979C was susceptible to several antibiotics and did not produce histamine or beta-haemolysis in blood agar containing red blood cells from various origins.ConclusionThe results demonstrated that L. fermentum UCO-979C is a very good candidate as a probiotic for the protection of humans against H. pylori infections.