A liquid chromatography-mass spectrometric method for the detection of cyclic β-amino fatty acid lipopeptides
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- 作者：Petra Urajová ; a ; Jan Há ; jeka ; b ; c ; Matti Wahlstend ; Jouni Jokelad ; Tomá ; &scaron ; Galicaa ; b ; David P. Fewerd ; Andreja Kusta ; b ; c ; Eli&scaron ; ka Zapomělová ; -Kozlí ; ková ; c ; Kateřina Delawská ; a ; b ; Kaarina Sivonend ; Jiří ; Kopecký ; a ; b ; Pavel Hrouzeka ; b ; hrouzekp@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lipopeptides ; Bacteria ; Cyanobacteria ; LC-HRMS ; Fatty acid ; Peptide
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：18 March 2016
- 年：2016
- 卷：1438
- 期：Complete
- 页码：76-83
- 全文大小：1128 K
文摘
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A selective method for the identification of bacterial cyclic β-amino fatty acid lipopeptides.
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Chromatographic separation coupled with high resolution mass spectrometry analysis at varying collision energies allows the determination of both amino-acid sequence and the presence of β-amino fatty acids.
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β-amino fatty acid immonium ions can be used as diagnostic fragments for selected lipopeptides.
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A linear relationship between length of fatty acid and retention time within linear gradient was confirmed.
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The method allows the detection of lipopeptide variants, including minor congeners, in comparison to non-targeted methods.