IBDV particles packaged with only segment A could be obtained.
IBDV-A particles supports random assortment mechanism for genomic packaging.
Our data also suggested the packaging elements of segment B contain 5′ and 3′ NCR.
Additional packaging signal other than NCRs was required for IBDV genomic packaging.
The formation of ribonucleoprotein (RNP) complexes might be involved in the assembly of viral particles.