Oxidative stress and delayed-onset muscle damage after exercise
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- 作者：Aoi ; Wataru ; Naito ; Yuji ; Takanami ; Yoshikazu ; Kawai ; Yukari ; Sakuma ; Kunihiro ; Ichikawa ; Hiroshi ; et. al.
- 关键词：Reactive oxygen species ; Exercise ; Muscle damage ; Inflammation ; Rat ; α ; -Tocopherol ; Free radicals ; AP-1 ; activator protein-1 ; CINC-1 ; cytokine-induced neutrophil chemoattractant-1 ; DMEM ; Dulbecco's modified Eagle's medium ; ELISA ; enzyme-liked immunoso
- 刊名：Free Radical Biology and Medicine
- 出版年：2004
- 出版时间：August 15, 2004
- 年：2004
- 卷：37
- 期：4
- 页码：480-487
- 全文大小：387 K
文摘
Reactive oxygen species (ROS) produced during exercise may be involved in delayed-onset muscle damage related to inflammation. To investigate this hypothesis, we studied whether oxidative stress increases nuclear translocation of nuclear factor-κB and chemokine expression in skeletal muscle using myotube L6 cells. We also assessed whether prolonged acute exercise could increase these parameters in rats. In L6 cells, H2O2 induced nuclear translocation of p65 and increased the expression of cytokine-induced neutrophil chemoattractant-1 (CINC-1) and monocyte chemoattractant protein-1 (MCP-1), whereas preincubation with α-tocopherol limited the increase in these proteins. Sprague Dawley rats were divided into the following groups: rested control, exercised, rested with a high α-tocopherol diet, and exercised with a high α-tocopherol diet. After 3 weeks of acclimation, both exercise groups ran on a treadmill at 25 m/min for 60 min. Exercise increased nuclear p65, CINC-1, and MCP-1 in gastrocnemius muscle cells, but these changes were ameliorated by the high α-tocopherol diet. Increases in myeloperoxidase and thiobarbituric acid-reactive substrates were ameliorated by a high α-tocopherol diet, as were the histological changes. Neutrophil activity was not altered by either exercise or a high α-tocopherol diet. These results indicate that delayed-onset muscle damage induced by prolonged exercise is partly related to inflammation via phagocyte infiltration caused by ROS and that α-tocopherol (an antioxidant) can attenuate such inflammatory changes.