Expression and methylation data from SLE patient and healthy control blood samples subdivided with respect to ARID3a levels

详细信息    查看全文

• 作者：Julie M. Ward^a ; ^{julie-ward@ouhsc.edu} ; Michelle L. Ratliff^a ; ^{michelle-ratliff@ouhsc.edu} ; Mikhail G. Dozmorov^b ; ^{Mikhail.dozmorov@vcuhealth.org} ; Graham Wiley^c ; ^{graham-wiley@omrf.org} ; Joel M. Guthridge^c ; ^{joel-guthridge@omrf.org} ; Patrick M. Gaffney^c ; ^{patrick-gaffney@omrf.org} ; Judith A. James^a ; ^c ; ^d ; ^{judith-james@omrf.org} ; Carol F. Webb^a ; ^e ; ^f ; ^{carol-webb@ouhsc.edu}
• 关键词：SLE ; B cells ; ARID3a
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：9
• 期：Complete
• 页码：213-219
• 全文大小：244 K
• 卷排序：9

文摘

Previously published studies revealed that variation in expression of the DNA-binding protein ARID3a in B lymphocytes from patients with systemic lupus erythematosus (SLE) correlated with levels of disease activity (“Disease activity in systemic lupus erythematosus correlates with expression of the transcription factor AT-rich-interactive domain 3A” (J.M. Ward, K. Rose, C. Montgomery, I. Adrianto, J.A. James, J.T. Merrill et al., 2014) [1]). The data presented here compare DNA methylation patterns from SLE peripheral blood mononuclear cells obtained from samples with high numbers of ARID3a expressing B cells (ARID3aH) versus SLE samples with normal numbers of ARID3a+ B cells (ARID3aN). The methylation data is available at the gene expression omnibus (GEO) repository, “Gene Expression Omnibus: NCBI gene expression and hybridization array data repository” (R. Edgar, M. Domrachev, A.E. Lash, 2002) [2]. Isolated B cells from SLE ARID3aH and ARID3aN B samples were also evaluated via qRT-PCR for Type I interferon (IFN) signature and pathway gene expression levels by qRT-PCR. Similarly, healthy control B cells and B cells stimulated to express ARID3a with the TLR agonist, CpG, were also compared via qRT-PCR. Primers designed to detect 6 IFNa subtype mRNAs were tested in 4 IFNa, Epstein-Barr Virus-transformed B cell lines (“Reduced interferon-alpha production by Epstein-Barr virus transformed B-lymphoblastoid cell lines and lectin-stimulated lymphocytes in congenital dyserythropoietic anemia type I” (S.H. Wickramasinghe, R. Hasan, J. Smythe, 1997) [3]). The data in this article support the publication, “Human effector B lymphocytes express ARID3a and secrete interferon alpha” (J.M. Ward, M.L. Ratliff, M.G. Dozmorov, G. Wiley, J.M. Guthridge, P.M. Gaffney, J.A. James, C.F. Webb, 2016) [4].