Identification of amino acid residues of mammalian mitochondrial phosphate carrier important for its functional expression in yeast cells, as achieved by PCR-mediated random mutation and gap-repair cloning

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• 作者：Ryohei Yamagoshi^a ; ^b ; Takenori Yamamoto^a ; ^b ; Mitsuru Hashimoto^c ; Ryohei Sugahara^d ; Takahiro Shiotsuki^d ; Hideto Miyoshi^e ; Hiroshi Terada^f ; Yasuo Shinohara^a ; ^b ; ^{yshinoha@genome.tokushima-u.ac.jp}
• 关键词：PiC ; mitochondrial phosphate carrier ; hPiC ; PiC of human ; rPiC ; PiC of rat ; yPiC ; PiC of yeast ; ΔNrPiC ; PiC lacking its presequence
• 刊名：Mitochondrion
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：32
• 期：Complete
• 页码：1-9
• 全文大小：1393 K
• 卷排序：32

文摘

We tried to express mammalian mitochondrial phosphate carrier in yeast cells. Rat protein lacking its presequence was well expressed in yeast, but it was inert. For functional expression, point mutation of either F267S or F282S, was required. These results were also observed with human carrier protein.