A new graphic plot analysis for determination of neuroreceptor binding in positron emission tomography studies

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• 作者：Hiroshi Ito ; Takashi Yokoi ; Yoko Ikoma ; Miho Shidahara ; Chie Seki ; Mika Naganawa ; Hidehiko Takahashi ; Harumasa Takano ; Yuichi Kimura ; Masanori Ichise ; Tetsuya Suhara
• 刊名：NeuroImage
• 出版年：2010
• 出版时间：1 January 2010
• 年：2010
• 卷：49
• 期：1
• 页码：578-586
• 全文大小：884 K

文摘

In positron emission tomography (PET) studies with radioligands for neuroreceptors, tracer kinetics have been described by the standard two-tissue compartment model that includes the compartments of nondisplaceable binding and specific binding to receptors. In the present study, we have developed a new graphic plot analysis to determine the total distribution volume (V_T) and nondisplaceable distribution volume (V_ND) independently, and therefore the binding potential (BP_ND). In this plot, Y(t) is the ratio of brain tissue activity to time-integrated arterial input function, and X(t) is the ratio of time-integrated brain tissue activity to time-integrated arterial input function. The x-intercept of linear regression of the plots for early phase represents V_ND, and the x-intercept of linear regression of the plots for delayed phase after the equilibrium time represents V_T. BP_ND can be calculated by BP_ND = V_T / V_ND − 1. Dynamic PET scanning with measurement of arterial input function was performed on six healthy men after intravenous rapid bolus injection of [¹¹C]FLB457. The plot yielded a curve in regions with specific binding while it yielded a straight line through all plot data in regions with no specific binding. V_ND, V_T, and BP_ND values calculated by the present method were in good agreement with those by conventional non-linear least-squares fitting procedure. This method can be used to distinguish graphically whether the radioligand binding includes specific binding or not.