An alumina (α-Al2O3) bicrystal with a {1 0 0}/1 1 0 2° low-angle grain boundary was fabricated by diffusion bonding, and the grain boundary was observed by high-resolution transmission electron microscopy (HRTEM). It was found that 1/31 0 0 partial-dislocation triplets were periodically arrayed along the boundary. The atomic structure within the {1 0 0} stacking faults in between the partial-dislocation triplets was determined by HRTEM combined with first-principles calculations. We discuss the stacking fault structures and their excess energies in detail.