A novel cyanobiphenyl benzothiazole-based fluorescent probe for detection of biothiols with a large Stokes shift and its application in cell imaging

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• 作者：Song Chen ; Hongmei Li ; Peng Hou ; ^{houpeng1982@163.com}
• 关键词：ESIPT ; Fluorescent probe ; Cell imaging ; Thiols ; PET
• 刊名：Tetrahedron
• 出版年：2017
• 出版时间：2 February 2017
• 年：2017
• 卷：73
• 期：5
• 页码：589-593
• 全文大小：1104 K
• 卷排序：73

文摘

An ESIPT-based fluorescent probe (Probe 1) with a large Stokes shift (202 nm) for the sensing of biothiols has been developed based on cyanobiphenyl benzothiazole derivative exhibiting larger Stokes shift than 2-(benzothiazol-2-yl)phenol. This probe with 2,4-dinitrobenzenesulfonate (DNBS) as a highly thiol-selective group was constructed based on the combination of excited state intramolecular proton transfer (ESIPT) and photoinduced electron transfer (PET) mechanisms. Upon the treatment with biothiols, this probe produced a remarkable fluorescence enhancement at 482 nm. The detect limits for Cys, GSH and Hcy was calculated to be as low as 2.0 × 10−8 M, 1.7 × 10−7 M and 1.2 × 10−7 M, respectively (based on S/N = 3). Importantly, application of this probe was successfully demonstrated by imaging thiols in living NCI-H226 cells.