Two distinct human POM121 genes: Requirement for the formation of nuclear pore complexes
详细信息 查看全文
- 作者：Tomoko Funakoshi ; Kazuhiro Maeshima ; Kazuhide Yahata ; Sumio Sugano ; Fumio Imamoto ; Naoko Imamoto
- 关键词：Nuclear pore complex ; Pom121 ; Nuclear structure ; RNAi ; Segmental-duplication
- 刊名：FEBS Letters
- 出版年：2007
- 出版时间：16 October 2007
- 年：2007
- 卷：581
- 期：25
- 页码：4910-4916
- 全文大小：1668 K
文摘
Pom121 is one of the integral membrane components of the nuclear pore complex (NPC) in vertebrate cells. Unlike rodent cells carrying a single POM121 gene, human cells possess multiple POM121 gene loci on chromosome 7q11.23, as a consequence of complex segmental-duplications in this region during human evolution. In HeLa cells, two “full-length” Pom121 are transcribed and translated by two distinct genetic loci. RNAi experiments showed that efficient depletion of both Pom121 proteins significantly reduces assembled NPCs on nuclear envelope. Pom121-depletion also induced clustering of NPCs, indicating its role on maintenance of NPC structure/organization.