Oral administration of attenuated Salmonella typhimurium containing a DNA vaccine against rabbit haemorrhagic disease

详细信息    查看全文

• 作者：Li Qiu ; Xinglong Wang ; Huafang Hao ; Guohui Mu ; Ruyi Dang ; Jia Wang ; Shuxia Zhang ; Enqi Du ; Zengqi Yang ; ^{nwafucrb@gmail.com}
• 关键词：S. typhimurium ; DNA vaccine ; RHDV ; Immune response
• 刊名：Journal of Virological Methods
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：188
• 期：1-2
• 页码：108-113
• 全文大小：459 K

文摘

The use of attenuated Salmonella typhimurium as a bactofection vehicle for the oral delivery of a DNA vaccine against rabbit haemorrhagic disease virus (RHDV) was investigated. The DNA vaccine plasmid pcDNA3.1-VP60, which encodes the viral capsid protein VP60, was transformed into the attenuated S. typhimurium strain SL7207. The resulting recombinant bacteria, named as SL/pcDNA3.1-VP60, were orally used to immunise rabbits. The successful delivery of the DNA plasmid was confirmed by the detected VP60 transcription in the rabbit intestines through the reverse transcription polymerase chain reaction. In addition, the RHDV-specific humoral and cell-mediated immune response that was induced by SL/pcDNA3.1-VP60 was detected by the enzyme-linked immunosorbent assay as well as the assays for T lymphocyte proliferation and cytokines secretion. The significant protection of immunised rabbits against the RHDV strain XA/China/2010 at 42 d post-immunisation was demonstrated. This study is the first report about the efficient usage of attenuated Salmonella as a live vector for the oral delivery of a DNA vaccine against RHDV.