Utilizing immunoaffinity chromatography (IAC) cross-reactivity in GC-MS/MS exemplified at the measurement of prostaglandin E1 in human plasma using prostaglandin E2-specific IAC columns
详细信息 查看全文
- 作者：Dimitrios Tsikasa ; tsikas.dimitros@mh-hannover.de" class="auth_mail" title="E-mail the corresponding author ; Maria-Theresia Suchya ; Klaus Tö ; dterb ; Joerg Heerenb ; Ludger Schejab
- 关键词：Cross-reactivity ; Immunoaffinity column chromatography ; Metabolic disorder ; Stable isotopes ; Tandem mass spectrometry
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：1021
- 期：Complete
- 页码：101-107
- 全文大小：1335 K
文摘
- •
Prostaglandin E1 (PGE1) is produced from DGLA by cyclooxygenase.
- •
PGE1 was quantitated in human plasma by GC–MS/MS.
- •
PGE1 was extracted by IAC-PGE2 (cross reactivity 18%).
- •
PGE1 was found to correlate with DGLA.