Development of new quantitative mass spectrometry and semi-automatic isofocusing methods for the determination of Apolipoprotein E typing
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- 作者：Christophe Hirtza ; 1 ; Jerome Vialareta ; 1 ; Georges Nouadjeb ; Susanna Schraenc ; Pascale Benliand ; e ; Sandrine Maryd ; Pascal Philibertf ; Laurent Tiersa ; Pauline Brosa ; Constance Delabya ; Audrey Gabellea ; g ; Sylvain Lehmanna ; s-lehmann@chu-montpellier.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Apolipoprotein E ; Mass spectrometry ; Isoelectric focusing ; Immunoprecipitation ; Immunofixation ; Phenotype ; Genotype ; Alzheimer's disease
- 刊名：Clinica Chimica Acta
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：454
- 期：Complete
- 页码：33-38
- 全文大小：775 K
文摘
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A comparison of two complementary techniques (quantitative mass spectrometry and isofocusing) for the phenotyping of Apolipoprotein E is proposed.
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Apolipoprotein E known to be a major risk factor for late-onset Alzheimer's and cardiovascular diseases
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These 2 methods allow the of use small volume (2 to 20 μL) of residual blood from samples collected for routine clinical tests, thus enabling retrospective studies with preserved body fluids.