Autophagy in the brains of young patients with poorly controlled T1DM and fatal diabetic ketoacidosis

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• 作者：William H. Hoffman ; John J. Shacka ; Anuska V. Andjelkovic
• 关键词：Autophagic gene-4 ; Cleaved caspase-3 ; Diabetic encephalopathy ; Diabetic ketoacidosis ; Glucose-regulated protein78 ; Light chain-3 ; Oxidative stress
• 刊名：Experimental and Molecular Pathology
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：93
• 期：2
• 页码：273-280
• 全文大小：761 K

文摘

Semi-quantitative neuroradiologic studies, quantitative neuron density studies and immunocytochemistry markers of oxidative stress and neuroinflammation indicate neuronal injury and deficits in young patients with chronic poorly controlled type 1 diabetes mellitus (T1DM). Present data suggest that pathogenesis of the neuronal deficits in young patients, who die as the result of diabetic ketoacidosis (DKA) and brain edema (BE), does not involve apoptosis, a prominent form of regulated cell death in many disease states. To further address this we studied mediators of macroautophagy, endoplasmic reticulum (ER) stress and apoptosis. In all areas studied we demonstrated increased levels of macroautophagy-associated proteins including light chain-3 (LC3) and autophagy related protein-4 (Atg4), as well as increased levels of the ER-associated glucose-regulated protein78/binding immunoglobulin protein (GRP78/BiP) in T1DM. In contrast, cleaved caspase-3 was rarely detected in any T1DM brain regions. These results suggest that chronic metabolic instability and oxidative stress may cause alterations in the autophagy-lysosomal pathway but not apoptosis, and macroautophagy-associated molecules may serve as useful candidates for further study in the pathogenesis of early neuronal deficits in T1DM.