Identification of peptides presented by HLA class I molecules on cervical cancer cells with HPV-18 infection

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• 作者：Monroy-Garcí ; a ; Alberto ; Ortí ; z-Navarrete ; Vianney Francisco ; Mora-Garcí ; a ; Marí ; a de Lourdes ; et. al.
• 关键词：Cervical cancer cells ; Human papillomavirus ; HLA class I bound peptides ; viral antigens ; β ; ₂m ; β ; ₂-microglobulin ; CTL ; cytotoxic T lymphocyte ; HCMV ; human cytomegalovirus ; HLA-A ; -B ; -C ; human leukocyte antigens ; HPV ; human p
• 刊名：Immunology Letters
• 出版年：1999
• 出版时间：April 15, 1999
• 年：1999
• 卷：67
• 期：3
• 页码：167-177
• 全文大小：609 K

文摘

In this work we eluted peptides from purified class I MHC molecules, isolated from a novel human cervical carcinoma cell line (INBL), generated in our laboratory and positive for HPV-18 infection. A fraction of these peptides was capable of stimulating T lymphocytes obtained from a donor matched for HLA-Cw4 and who was also HPV-18+. Direct N-terminal Edman degradation of these peptides, revealed the sequence (XQFPIFLQF) that matched 85 % with the sequence NVFPIFLQM localized in between the 54 and 62 residues of the HPV-18 L1 protein. After stimulation with the synthetic peptide NVFPIFLQM, T lymphocytes from the donor were capable to lyse INBL cells. Our results provide evidence of the existence of naturally occurring viral epitopes presented on cervical cancer cells by the HLA-Cw4 allele, that could be useful for immunotherapy on this type of patient.